You are to separate three chemicals (A, B, C) on a reverse phase HPLC column with a UV detector. You
You are to unconnected three chemicals (A, B, C) on a reverse countenance HPLC post after a while a UV undeceiver. You foremost practised isocratic issue after a while a mobile countenance of 10% acetonitrile and 90% of infiltrate at a issue rate of 1 mL/min. The grasp times at this predicament are 2.5, 5.5, and 5.9 minutes for chemical A, B, and C, respectively. Which chemical is most polar? Since the grasp times for chemical B and C are so cease and peaks are a slight overlapped, what would you do to amend unconnected these two compounds? Why?