Sci 115 microarray lab and quiz


  

Question 1 

  1. Most scientists who use DNA      microarrays obtain them 

  

from companies that lump consequence them   

 

from the government 

 

by making them in their labs 

 

by exploiting ambitious disequalize   students 

4 aims   

Question 2 

  1. "In this con-over, cancerous and      regular peel texture cases were excised delay a scalpel. Each peel case      was then placed into a case tube parallel delay solvent, oscillaten on a struggle      and centrifuged. When the case tubes came out of the centrifuge, there      was a top flake and a groundwork flake. For our intentions, we went on to use      ______________ for elevate mannering." 

  

only the top flake 

 

only the groundwork flake 

 

a structure of top and groundwork flakes 

 

none of the options listed 

4 aims   

Question 3 

  1. The intention of the struggle      instrument is to 

  

vigorously oscillate the texture case in   the solvent so that it dissolves. 

 

spin the texture/solvent structure   around to unconnected the solids 

 

action the mRNA to oblige to the pellets   

 

dispose of biohazardous wane safely 

3 aims   

Question 4 

  1. "Once the RNA is unadorned      from the DNA, proteins and other materials, the solvent/RNA structure is run      through a shaft of all elaborates. Each elaborate has sundry less strands of      polyT RNA holding out of it. In this step:" 

  

"mRNA holds to the elaborates, time   rRNA and tRNA run fit through the shaft" 

 

"tRNA holds to the elaborates, time   mRNA and rRNA run fit through the shaft" 

 

"rRNA and tRNA hold to the   beads, time mRNA runs fit through the shaft" 

 

none of the options listed 

4 aims   

Question 5 

  1. "Once the mRNA is unadorned,      you fashion a DNA representation of it using by adding polyT primers, labeled DNA      nucleotides, and an enzyme unreserved as:" 

  

reverse imagease 

 

tyrosine hydroxylase 

 

alcohol dehydrogenase 

 

ATP kinase 

4 aims   

Question 6 

  1. A DNA representation of a mRNA image is      unreserved as 

  

complementary DNA (abbreviated cDNA) 

 

copied DNA (abbreviated cDNA) 

 

mitochondrial DNA (abbreviated mtDNA)   

 

recombinant DNA (abbreviated rDNA) 

3 aims   

Question 7 

  1. Each flaw on the DNA microadorn in      embedded delay 

  

copies of DNA from one detail   gene 

 

copies of DNA from sundry contrariant   genes delay common functions 

 

copies of DNA from sundry contrariant   genes delay contrariant functions 

 

copies of mRNA from one or over genes   

4 aims   

Question 8 

  1. "When we say that the cDNA      derived from our case has hybridized to a detail flaw on the adorn,      we moderation that: " 

  

The cDNA from the case has stuck to   the DNA on the microadorn at that aim. 

 

The cDNA from the case is mixed   and cannot be trusted. 

 

The cDNA has been used to fashion an   mRNA representation 

 

You insufficiency to buy microarrays from   contrariant manufacturers and triangulate the results 

4 aims   

Question 9 

  1. "In this detail      experiment, we used red-labeled DNA to manner the case from      _____________ texture and the untried-labeled DNA to manner the case from      ___________. " 

  

cancerous; regular 

 

normal; cancerous 

 

normal; regular 

 

cancerous; cancerous 

3 aims   

Question 10 

  1. "After scanning the untried      labeled areas and the red labeled areas, when we connect the two images,      the flaws that profession up as yellow match to " 

  

genes explicit by twain regular or   cancerous peel cells. 

 

genes that were not explicit at all   in either regular or cancerous peel cells 

 

"genes explicit by cancerous,   but not regular peel cells" 

 

"genes explicit by regular, but   not cancer peel cells. " 

4 aims   

Question 11 

  1. "In interpreting the results      of this con-over, flaws on the microadorn that are red match to:" 

  

genes that are acid up by cancer 

 

genes that are acid down by cancer 

 

genes that are simple by cancer 

 

genes that aren t explicit in regular   or cancerous cells 

4 aims   

Question 12 

  1. "In interpreting the results      of this con-over, flaws on the the microadorn that are untried match to      genes that are " 

  

genes that are acid down by cancer 

 

genes that are acid up by cancer 

 

genes that are simple by cancer 

 

genes that aren t explicit in regular   or cancerous cells 

3 aims   

Question 13 

  1. "When the DNA microadorn      con-over tells us that a comprehensive reckon of genes feel been acid up (or acid      down) by a empiricism, the most slight interpretation is that " 

  

the acid up genes are slight   controlled by a gene that has bybypast bad 

 

all of these genes are genes that   feel bybypast bad themselves 

 

even proper one rear in any of   these improbable genes would feel been competent to action the empiricism 

 

the cytoplasm has too frequent unconditional   radicals in it 

4 aims   

Question 14 

  1. A gene professionn by the microadorn to      be explicit is : 

  

"probably making protein, but a   protein indication segregation would be insufficiencyed to distinguish for sure" 

 

definitely making protein 

 

definitely not making protein 

 

probably making protein but there's   no way to distinguish for sure 

4 aims   

Question 15 

  1. "In the contact of this      technique to peel cancer, a gene that has bybypast bad " 

  

"may or may not be attested at   all, but the overall shape of results can confer essential clues" 

 

can approximately constantly be attested via   DNA microarray