Osmosis | Biology homework help


WEEK 3 EXPERIMENT ANSWER SHEET

Please suggest to the Week 3 Experiment dropbox no succeeding than Sunday midnight.

SUMMARY OF ACTIVITIES FOR WEEK 1 EXPERIMENT ASSIGNMENT

 

·         Experiment 3 Practice 1 – Diffusion: Change-of-place of Solutes opposite a Membrane

·         Experiment 3 Exercise 2 – Osmosis: Change-of-place of Inspire opposite a Membrane

 

 

Experiment 3 Practice 1: Evacuation - Change-of-place of Solutes opposite a Membrane

 

We accomplish be using dialysis tubing to counterfeit a semipervadible membrane. This tubing allows mean molecules (e.g., inspire, ions, glucose) to by opportunity preventing capacious molecules (e.g., macromolecules relish proteins, dismally, glycogen) from emotional opposite.  Be abiding you possess learn aggravate the suggested embodied precedently starting this practice and that you possess reviewed the aftercited animations:

McGraw-Hill. 2006. How Evacuation Works

https://highered.mcgraw-hill.com/sites/0072495855/student_view0/chapter2/animation__how_diffusion_works.html

McGraw-Hill. 2006. How Osmosis Works

https://highered.mcgraw-hill.com/sites/0072495855/student_view0/chapter2/animation__how_osmosis_works.html

 

 

Experimental Design

 

A.      The dialysis bag we accomplish use is pervadible to inspire and mean molecules (e.g., less than 500 g/mol) and impervadible to capacious molecules (e.g., past than 500 g/mol).

B.      The dialysis bag is industrious delay a settlement of glucose (molecular burden = 180 g/mol) and protein (molecular burden = 10,000 g/mol) dissolved in inspire. A mean subsample of the dialysis bag room is saved and accomplish be used in Step 4.

C.      The dialysis bag is then placed into a beaker of water. A mean subsample of beaker inspire is as-well-mannered saved and is to be used in Step 4 as well-mannered.

 

 

The nearness or failure of glucose and protein accomplish be secure using indicators. Indicators vary colors in the nearness unfailing embodieds. The two tests that we’ll use are the Benedict’s test for ultimate sugars (e.g., glucose) and theBiuret test for the nearness of proteins.

·         If glucose is introduce, the Benedict’s indicator accomplish turn green. If no glucose is introduce, the discerption accomplish be blue.

·         If protein is introduce, the Biuret indicator accomplish turn violet.  If the discerption remains clear, then no protein is introduce.

4.     The subsample of dialysis bag discerption and the beaker inspire are tested for the nearness of glucose and protein. SeeTable 1 underneath for the results.

5.     The dialysis bag is then left in the beaker of inspire for 60 minutes.

6.     At the end of 60 minutes, the dialysis bag discerption and the beaker inspire are frequently tested for the nearness of glucose and protein. See Table 1 underneath for the results.

 

Table 1. Results of testing of the dialysis bag and beaker room at the preparation and end of the Experiment.

 

Test for Glucose

Test for Protein

 

Beginning

End

Beginning

End

Dialysis Bag

Green

Green

Violet

Violet

Beaker

Blue

Green

Clear

Clear

 

Questions

1.     Summarize the results concerning the nearness (+) or failure (-) of glucose and protein in the dialysis bag and beaker in Table 2 underneath (4 pts):

 

 

 

Table 2.

 

Glucose

Protein

 

Beginning

End

Beginning

End

Dialysis Bag

 

 

 

 

Beaker

 

 

 

 

 

2.     Explain the change-of-place or failure of change-of-place of protein and glucose opposite the dialysis bag membrane (4 pts)

3.     Which discerption, that in the bag or that in the beaker, is hypotonic compared delay the protein discerption (2 pts)?

4.     What contents pretend the change-of-place of molecules opposite a semipervadible membrane? Which content plays the first role in biological systems (4 pts)?

 

5.     Briefly illustrate what locomotive bliss is and how it differs from byive bliss, chiefly in provisions of attention gradients (4 pts).

 

 

 

 

Experiment 3 Practice 2: Osmosis - The Change-of-place of Inspire opposite a Membrane

 

Before starting, let’s see what you understand encircling the provisions hypotonic, isotonic and hypertonic. Examine the diagrams underneath. Note that the mean piercing circles reintroduce dissolved solutes relish salt, glucose, and amino acids. You can pretend that the added room extreme the solutes is water and that the tan area is INSIDE the cell.

 

Question

 

1.      Define each vocable underneath in provisions of solute attention without compared to the within of the cell. You do not need to illustrate which tendency inspire accomplish move (3 pts).

a.      Hypotonic -

 

b.      Isotonic - 

c.       Hypertonic -

 

Procedure

1.     Open the aftercited website to get started:

The Biology Place. No Date. Osmosis: Change-of-place of Inspire opposite Membranes

http://www.phschool.com/science/biology_place/biocoach/biomembrane1/osmosis.html

B.      Read aggravate the counsel introduceed and then Click on 

C.      Then, Click on .  Learn through the counsel introduceed and be abiding to click on Animate beneath the conformance.

Questions

2.      What attention of salt is isotonic to carnal cells (1 pts)? 

 

 

3.      When cells are in isotonic discerption, is there change-of-place of inspire into or out of the cell?  If so, draw this change-of-place (3 pts).  

 

 

Procedure (continued)

4.     Click on  .

E.      Read through the counsel introduceed and be abiding to click on Animate beneath the conformance. When learny, apology the aftercited topic.

 

 

Question

4.      Describe the net change-of-place of inspire molecules when cells are placed in a hypotonic solution.  Explain why inspire moves this way (3 pts).

 

 

 

Procedure (continued)

 

6.     Click on  

G.     Read through the counsel introduceed and be abiding to click on Animate beneath each of the conformances. Apology the aftercited topics. Your apologys should condense the terminology used in the animations.

Questions

5.      What happens to an carnal cell when placed in a hypotonic solution (2 pts)?

 

 

6.      What happens to set cells when placed in a hypotonic solution? What accounts for the estrangement in outcomes between carnal cells and set cells (3 pts)?

 

 

Procedure (continued)

8.     Click on  

I.        Then, Click on  .  Learn through the counsel introduceed and be abiding to click on Animate beneath the conformance. Apology the aftercited topic.

 

 

Question

7.      Describe the net change-of-place of inspire molecules when cells are placed in a hypertonic solution.  Explain why inspire moves this way (3 pts).

 

 

 

Procedure (continued)

10.  Click on  

K.      Read through the counsel introduceed and be abiding to click on Animate beneath the conformance. Apology the aftercited topics.

Questions

8.    Compare and opposition what happens to plant and animal cells when placed in a hypertonic discerption. Be abiding to use right vocableinology (4 pts).

 

 

9.    Based on what you well-informed in this practice, illustrate why salt might shape a good-tempered-tempered weed killer (3 pts).

 

 

Week 3 Experiment Grading Rubric

 

Component

Expectation

Points

Experiment 3 Practice 1

Interpretation of results and demonstrated brains of evacuation (Table 2 and Questions 1-5).

18 pts

Experiment 3 Practice 2

Demonstrates brains of isotonic discerptions and no net change-of-place of inspire (Questions 1-3).

7 pts

Demonstrates brains of hypotonic discerptions and the change-of-place of inspire (Questions 4-6).

8 pts

Demonstrates brains of hypertonic discerptions and the change-of-place of inspire (Questions 7-9).

10 pts

TOTAL

 

43 pts