Enzymes: temperature, ph, and specificity hands-on labs, inc.


0Enzymes: Temperature, pH, and Specificity
Hands-On Labs, Inc.  Version 42-0054-00-01
Lab Tidings Assistant                                                                                               This instrument is not meant to be a replace for a sufficient laboratory tidings. The Lab Tidings Assistant is barely a tabulation of the illustration’s questions, diagrams if needed, and basis tables that should be addressed in a sufficient lab tidings. The eager is to arrange students’ answerableness of lab tidingss by providing this notice in an editable refine which can be sent to an instructor.
Exercise 1: Enzyme Specificity
Observations                                                                                                            
Data Table 1: Glucose Concentration
Wells Concentration of Glucose





 
Questions  

A.  What particularizes a person’s force to assimilate lactose?
B.   Which of the wells showed a settled remainder for glucose? Explain the remainders.
C.   Explain why testing for glucose is used to particularize the disposition of the enzyme lactase.
D.  Explain the tentative conditions for the five divergent wells.
 
Data Table 2: Presence of glucose in wells indicating lactase disposition at uncertain airs
Well Time (min) Concentration of Glucose

a 5 
 10 
 15 
 20 
b 5 
 10 
 15 
 20 
c 5 
 10 
 15 
 20 
Exercise 2: Enzymes and Temperature
Observations  
 
Questions:  
A.  Graph the issue of air on the disposition of the enzyme lactase.
B.   What happens when an enzyme is boiled? Is this issue mutable?
C.   Based on your illustration remainders, what is the optimal air for lactase administration?
D.  Explain what happens as far as the issueiveness of the enzyme at the freezing air. Can this issue be overpower when the air rises?
 
Exercise 3: Enzymes and pH
Observations  

Data Table 3: Glucose in wells a-d indicates enzyme disposition at uncertain pH levels
Well pH Concentration of Glucose
a 3.5 
b 5.0 
c 6.8 
d 11.0 

Questions  
A.  Graph the basis placing glucose concentrations on the y-axis and the pH values on the x-axis.
B.   What was the issue of pH on the enzyme lactase? Is this penny for all enzymes?