Bs101 lab 6 microarray mcqs
1. Genomics is the consider of:
a. The texture and exercise of mutations and how they diversify genetic traits.
b. Genes and the DNA seriess betwixt genes and how they enumerate harvest.
c. The notice supposing by computer programs which analyzes mRNA.
d. The rational genome as paralleld to other vertebrate genomes.
2. Microarrays are a very accelerationful utensil in genomics consequently they:
a. Acceleration scientists discuss intergenetic DNA by separating it from genes.
b. Provide a choice countenancer tract-of-land for polymerase association reactions.
c. Tolerate scientists to discuss thousands of genes all at unintermittently.
d. Decrease the date it takes for scientists to fashion copies of DNA.
3. Generally, total cell in our organization contains the similar 20,000 (or so) genes. However, cells in our organization are opposed from each other consequently they:
a. Bear opposed genes crusty “on” or “off” to prop opposed exercises.
b. Contain opposed copies of genes for opposed exercises.
c. Provide opposed nucleotide disesteemeds for each harvestal exercise.
d. Exercise opposedly naturalized on varying proteomics.
4. How can scientists enumerate the exercise of or varietys betwixt cell signs? They can discuss the:
a. Number of nucleotide disesteemeds in genes versus intergenetic seriess.
b. Amount of mRNA explicit for each gene in a cell sign, and then parallel that notice betwixt cell signs.
c. Amount of mutations betwixt genes in the intergenetic spaces.
d. Number of tRNA copies for a point cell sign.
5. How is a microarray constrained? In each fault, there are:
a. Copies of all the genes for an organism.
b. Multiple copies of one gene; each fault has copies for a opposed gene.
c. Multiple copies of intergenetic seriess, which join to genes in the viewtlings.
d. Copies of intergenetic seriess, which aid the rejoinder of DNA in a viewtling.
6. The exemplification that begins in Chapter 3 of the hypocrisy seeks to confutation the question:
a. What is the variety betwixt intergenetic spaces in cancer cells versus salubrious cells?
b. Why do opposed cell signs specific opposed amounts of mRNA?
c. How do opposed cancer cells result opposed mutations?
d. What is the variety betwixt salubrious cells and cancer cells?
7. Why can’t doctors use cell advent to diagnose cancer?
a. Not all cancer cells observe opposed from salubrious cells.
b. Cancer cells are too feeble to discuss using cell advent.
c. Not all cancer cells are reciteing to be biopsied from the organization.
d. Cancer cells diversify advent when taken out of the organization.
8. In the exemplification, a solvent is assumed to each cell sign (salubrious cells and cancer cells). After the viewtling tube containing each cell sign is qualified on the hurry, the RNA is disjoined from the pause of the viewtling in a centrifuge. Why does DNA decide to the foot of the tube and RNA doesn’t?
a. RNA is ample longer than DNA.
b. RNA is strong to proteins that acceleration it alight in elucidation.
c. DNA is strong to biomolecules that weigh it down and acceleration it decide to the foot.
d. DNA is ample longer than RNA.
9. What element does mRNA bear that tRNA and rRNA do not? mRNA always:
a. Contains a GABA box.
b. Contains a TATA series.
c. Ends after a while a G inferiority.
d. Ends after a while a poly-A inferiority.
10. How do the beads in the column detached mRNA from all other RNA? The beads contain:
a. Sequences that magnetically detached the mRNA.
b. A glue-like pith adventitious from spider webs.
d. A series of uracil’s that join to the Poly-A inferiority.
11. After you dissociate mRNA, you bear to fashion a DNA observation. Why can’t we impartial use mRNA?
a. DNA is ample further sttelling than mRNA.
b. We bear to add a fluorescent label that earn tolerate us to see the viewtling.
c. mRNA earn eventually transfigure into tRNA making it unusable.
d. A and B
12. Scientists allure hybridization the key to microarrays. Hybridization occurs when:
a. Two panegyrical strands of DNA from opposed sources join to each other.
b. Poly-A inferioritys join to Poly-Ts.
c. Opposed reputation interbreed and make new DNA disesteemed pairings.
d. Two strands of same DNA join after a whileout using the transmitted nucleotide pairs.
13. When you view the microarray in the viewner, the facts pomp some black faults. What do these portray?
a. The DNA that has been replicated in salubrious cells.
b. The mRNA that was washed abroad in the washing elucidation.
c. The DNA that was not transcribed and explicit in salubrious cells.
d. The mRNA that was not frisk by Oligo-d-tails in the beads.
14. When you view the microarray in the viewner, some faults are yellow and portray places where the gene was explicit in twain salubrious and cancer cells. These faults recite us:
a. Where to observe for mutations.
b. Where DNA hybridized in cancer cells.
c. That DNA specificion didn’t diversify in these genes when cancer occurred.
d. That the microarray didn’t is-sue in these genes.
15. In our model, gene 6219 mRNA is made in twain salubrious and cancerous cells; eventually proteins are merely translated from that mRNA in salubrious cells. Microarray decomposition:
a. Shows us this fault by making yellow faults.
b. Cannot pomp us this fault, which is a constraint of this sign of decomposition.
c. Pomp us this fault by making red faults.
d. Cannot pomp us this fault, which is a good of this sign of decomposition.